Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus CirrhosisCorrespondence Address :
Dr. Abdolvahab Moradi,
Department of Microbiology, Faculty of Mcdicine, Golestan University of Medical Science, Gorgan-49177-65181, Golestan, Iran.
Introduction: Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease.
Aim: This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients.
Materials and Methods: Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing.
Results: Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775.
Conclusion: Considering the proposed role for some of these mutations in severe liver disease, further investigations are needed to clarify the importance of them in the early stages of hepatitis B infection.
Deletion mutation, Genotype D, Hepatitis B-virus, Hepatitis-B X-region, Insertion mutation
Farzaneh Salarnia, Sima Besharat, Sare Zhand, Naeme Javid, Behnaz Khodabakhshi, Abdolvahab Moradi. MUTATIONS IN HEPATITIS-B X-GENE
REGION: CHRONIC HEPATITIS-B
VERSUS CIRRHOSIS. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 Mar 28 ]; 11:OC31-OC34. Available from
Date of Submission: Jul 04, 2016
Date of Peer Review: Aug 30, 2016
Date of Acceptance: Nov 28, 2016
Date of Publishing: Mar 01, 2017
Financial OR OTHER COMPETING INTERESTS: None.
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