Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : March | Volume : 11 | Issue : 3 | Page : OC31 - OC34

Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis

Farzaneh Salarnia, Sima Besharat, Sare Zhand, Naeme Javid, Behnaz Khodabakhshi, Abdolvahab Moradi

1. Faculty, Infectious Disease Research Center, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 2. Faculty, PhD, Golestan Research Center of Gastroentrology and Hepatology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 3. Faculty, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 4. Faculty, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 5. Faculty, Golestan Research Center of Gastroentrology and Hepatology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 6. Faculty, Golestan Research Center of Gastroentrology and Hepatology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran.

Correspondence Address :
Dr. Abdolvahab Moradi,
Department of Microbiology, Faculty of Mcdicine, Golestan University of Medical Science, Gorgan-49177-65181, Golestan, Iran.
E-mail: abmoradi@yahoo.com

Abstract

Introduction: Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease.

Aim: This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients.

Materials and Methods: Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing.

Results: Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775.

Conclusion: Considering the proposed role for some of these mutations in severe liver disease, further investigations are needed to clarify the importance of them in the early stages of hepatitis B infection.

Keywords

Deletion mutation, Genotype D, Hepatitis B-virus, Hepatitis-B X-region, Insertion mutation

How to cite this article :

Farzaneh Salarnia, Sima Besharat, Sare Zhand, Naeme Javid, Behnaz Khodabakhshi, Abdolvahab Moradi. MUTATIONS IN HEPATITIS-B X-GENE REGION: CHRONIC HEPATITIS-B VERSUS CIRRHOSIS. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 Mar 28 ]; 11:OC31-OC34. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=March&volume=11&issue=3&page=OC31-OC34&id=9498

DOI and Others

DOI: 10.7860/JCDR/2017/22570.9498

Date of Submission: Jul 04, 2016
Date of Peer Review: Aug 30, 2016
Date of Acceptance: Nov 28, 2016
Date of Publishing: Mar 01, 2017

Financial OR OTHER COMPETING INTERESTS: None.

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