Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : March | Volume : 11 | Issue : 3 | Page : NC01 - NC04

Effect of Intraperitoneal Injection of Saffron on the Treatment of Experimental Endotoxin Induced Uveitis in the Rabbit

Mohammad Reza Talebnejad, Kazem Soltanzadeh, Eghtedari Masoomeh, Masoud Yasemi, Mohammad Reza Khalili, Sherafat Maryam

1. Professor, Department of Ophthalmology, Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Ophthalmologist, Department of Ophthalmology, Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Associate Professor, Department of Ophthalmology, Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Ophthalmologist, Department of Ophthalmology, Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Assistant Professor, Department of Ophthalmology, Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 6. Faculty, Department of Traditional Persian Medicine, School of Traditional Persian Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Correspondence Address :
Dr. Masoud Yasemi,
Ophthalmologist, Department of Ophthalmology, Poostchi Eye Research Center, Shiraz University of Shiraz, Fars, Iran.
E-mail: masoodyasemi@yahoo.com

Abstract

Introduction: Saffron with scientific name of “Crocus sativus” from the family “Iridaceae” is a plant without stem and root. Antioxidative and immunomodulatory effects of saffron has been demonstrated in different studies.

Aim: To assess the effects of saffron in the treatment on Lipopolysaccharide (LPS) induced uveitis in the rabbit.

Materials and Methods: Thirty healthy female New Zealand white rabbits were prepared. LPS induced uveitis was confirmed via slit lamp examination 24 hours after intravitreal injection of Salmonella typhimurium LPS endotoxin in the right eye of all rabbits. Then the animals were randomly divided into two equal groups - group 1 (controls) and group 2 (saffron). After 24 hours of intravitreal injection of LPS, 60 mg/body weight of saffron was injected intraperitoneally for Group 2. On the seventh day after intraperitoneal injection, all rabbits of both groups were euthanized with an overdose intraperitoneal injection of sodium thiopental. Immediately after euthanasia, enucleation of both groups of rabbits was done. Clinical Scoring System (CSS) and pathological severity score of uveitis was assessed for each eye.

Results: No statistical difference in the CSS was found in days 3,5, and 7 after intraperitoneal injection of saffron. Also, Pathological Severity Score (PSS) did not show a significant difference between two groups. Additionally, the maximum PSS of both groups was very closed but the number was observed in the saffron group (20%) more than control group (10%).

Conclusion: Intraperitoneal injection of saffron was ineffective in the management of experimental uveitis. Further studies with different dosages and follow up and using other routes of administration are needed.

Keywords

Crocus sativus, Immunomodulatory, Intravitral, Lipopolysaccharide

How to cite this article :

Mohammad Reza Talebnejad, Kazem Soltanzadeh, Eghtedari Masoomeh, Masoud Yasemi, Mohammad Reza Khalili, Sherafat Maryam. EFFECT OF INTRAPERITONEAL INJECTION OF SAFFRON ON THE TREATMENT OF EXPERIMENTAL ENDOTOXIN INDUCED UVEITIS IN THE RABBIT. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 Mar 28 ]; 11:NC01-NC04. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=March&volume=11&issue=3&page=NC01-NC04&id=9491

DOI and Others

DOI: 10.7860/JCDR/2017/23266.9491

Date of Submission: Aug 02, 2016
Date of Peer Review: Aug 26, 2016
Date of Acceptance: Nov 28, 2016
Date of Publishing: Mar 01, 2017

Financial OR OTHER COMPETING INTERESTS: None.

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