Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Experimental Research
Year : 2017 | Month : March | Volume : 11 | Issue : 3 | Page : FF01 - FF04

Evaluation of Acute Oral Toxicity of Ethanol Leaves Extract of Catharanthus roseus in Wistar Albino Rats

Venkateswar Rao Vutukuri, M.C.Das, Muralidhar Reddy, Siva Prabodh Padma Sunethri

1. Tutor, Department of Pharmacology, NRI Medical College, Chinakakani, Andhra Pradesh, India. 2. Professor and Head, Department of Pharmacology, NRI Medical College, Chinakakani, Andhra Pradesh, India. 3. Professor and Head, Department of Anatomy, NRI Medical College, Chinakakani, Andhra Pradesh, India. 4. Professor and Head, Department of Biochemistry, NRI Medical College, Chinakakani, Andhra Pradesh, India. 5. Assistant Professor, Department of Pathology, Gandhi Medical College, Hyderabad, Andhra Pradesh, India.

Correspondence Address :
Dr. Venkateswar Rao Vutukuri,
H no 5-2-247/H/3/2, Jana Sikshana Samsthan Road, Mustafa Nagar, Khammam-507001, Telangana, India.
E-mail: venkatsas111@gmail.com

Abstract

Introduction: Herbal drugs have gained importance globally in the treatment of various diseases. Catharanthus roseus is traditionally used as an anti diabetic and anti cancer drug. To the best of our knowledge limited data are available on acute and sub acute toxicity of C. roseus extract despite its wide spread use in traditional medicine. The present study was under taken to evaluate the acute oral toxicity of the ethanol leaves extract of C. roseus.

Aim: To investigate the acute oral toxic effects of ethanol leaves extract of C. roseus. The objective of the study was to evaluate organ toxicity related to heart, liver and kidney in wistar albino rats.

Materials and Methods: The experiment was done based on the Organization for Economic Co-operation and Development (OECD) guideline 420. The study included twenty five non pregnant female wistar rats (n=25). Sighting study was done using the fixed dose method. The study was conducted by giving a single dose of test drug varying from 5, 50, 300 and 2000 mg and animals were observed for fourteen days. Blood samples were collected and biochemical findings SGPT, SGOT, urea, creatinine, creatinine phosphokinase and Lactate Dehydrogenase (LDH) were analyzed. Food consumption, water intake and histopathology changes were evaluated.

Results: Catharanthus roseus Extract (CRE) at a dose of 2000 mg did not produce mortality. SGOT, SGPT, Creatinine phosphokinase, LDH, urea and creatinine were elevated in 300 mg and 2000 mg doses.

Conclusion: Though there was no mortality, the extract of C. roseus higher than 300 mg can produce signs of biochemical and histopathological toxicity in liver, kidney and heart. It is recommended that lower doses than the studied ones should be used for treatment.

Keywords

Biochemical analysis, Creatinine phosphokinase, Lactate Dehydrogenase, Herbal drugs, Histopathology

How to cite this article :

Venkateswar Rao Vutukuri, M.C.Das, Muralidhar Reddy, Siva Prabodh Padma Sunethri. EVALUATION OF ACUTE ORAL TOXICITY OF ETHANOL LEAVES EXTRACT OF CATHARANTHUS ROSEUS IN WISTAR ALBINO RATS. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 May 22 ]; 11:FF01-FF04. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=March&volume=11&issue=3&page=FF01-FF04&id=9325

DOI and Others

DOI: 10.7860/JCDR/2017/24937.9325

Date of Submission: Oct 20, 2016
Date of Peer Review: Nov 05, 2016
Date of Acceptance: Dec 08, 2016
Date of Publishing: Mar 01, 2017

Financial OR OTHER COMPETING INTERESTS: None.

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