Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology SmearsCorrespondence Address :
Dr. Hemalatha Anantharamaiah Shashidhar,
Associate Professor, Department of Pathology, Sri Devaraj Urs Medical College, Kolar-563101, Karnataka, India.
Introduction: Air dried cytology smears are stained routinely with Romanowsky stains so that the relative cell size, nuclear size, cytoplasmic details, smear background elements and intercellular matrix components are better appreciated. A variety of modified Romanowsky stains are used in cytology. Leishman-Giemsa (LG) cocktail is one of the new staining techniques which can be used for staining the air dried cytology smears.
Aim: To evaluate the quality of staining of LG cocktail on air dried smears and to compare the quality of staining of LG cocktail with May Grunwald Giemsa (MGG) which is the most commonly used stain in cytology.
Materials and Methods: The present prospective comparative study was carried out with 100 cases and two extra smears were prepared for each case and stained with MGG and LG cocktail stains. The stained slides were blinded and were evaluated for the staining characteristics of the nucleus, cytoplasm and background staining. Based on this, scoring was done by two pathologists independently. Quality Index (QI) was calculated by dividing the scores obtained with the total score possible.
Results: LG cocktail stained slides were excellent in cytoplasmic staining, granularity, nuclear morphology, background material staining and overall staining characteristics. QI of LG cocktail was 0.8 while that of MGG was 0.59.
Conclusion: Staining of air dried smears by LG cocktail has a good QI. It is also cheaper, requires short duration for staining as compared to MGG. Hence, LG cocktail can be an effective replacement for MGG for staining the air dried cytology smears.
May grunwald giemsa, Quality index, Romanowsky
Shilpa Manigatta Doddagowda, Hemalatha Anantharamaiah Shashidhar,
Chinaiah Subramanyam Babu Rajendra Prasad. LEISHMAN-GIEMSA COCKTAIL - IS IT AN
EFFECTIVE STAIN FOR AIR DRIED CYTOLOGY
SMEARS. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 May 25 ]; 11:EC16-EC18. Available from
Date of Submission: Nov 25, 2016
Date of Peer Review: Dec 11, 2016
Date of Acceptance: Jan 20, 2017
Date of Publishing: Mar 01, 2017
Financial OR OTHER COMPETING INTERESTS: None.
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