Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : April | Volume : 11 | Issue : 4 | Page : ZC94 - ZC96

Effect of Oral Tissue Fluids on Compressive Strength of MTA and Biodentine: An In vitro Study

Divya Subramanyam, Madhusudhan Vasantharajan

1. Postgraduate Student, Department of Paedodontics and Preventive Dentistry, Saveetha Dental College, Chennai, Tamil Nadu, India. 2. Senior Lecturer, Department of Paedodontics and Preventive Dentistry, Saveetha Dental College, Chennai, Tamil Nadu, India.

Correspondence Address :
Dr. Divya Subramanyam,
House no. 20, School Road, Venkateshwara Nagar, East Phase, Kodungaiyur-600118, Chennai, Tamil Nadu, India.


Introduction: Over the past many years various root end filling materials have been used which have been tested for their physical properties but each of them had certain limitations. In clinical practice, root end filling materials are exposed to oral tissue fluids which may compromise their longevity.

Aim: The aim of this study was to investigate the effects of oral tissue fluids on compressive strength of Mineral Trioxide Aggregate (MTA) and biodentine.

Materials and Methods: MTA and biodentine cylinders measuring 6 mm × 4 mm were prepared using acrylic blocks. They were divided into six groups; (Group 1) (MTA) (n=3), (Group 2) MTA contaminated with saliva, (MTA-S) (n=3), Group 3: MTA contaminated with blood, MTA-B (n=3), Group 4: Biodentine (BD), Group 5: Biodentine contaminated with saliva (BD-S) (n=5), Group 6: Biodentine contaminated with blood (BD-B) (n=5). The mould was contaminated with saliva and blood and incubated at 37°C at 100% humidity for three days and compressive strength (MPa) was measured using universal testing machine and the data was analyzed statistically using one-way ANOVA test.

Results: There was no significant difference in the compressive strength between the three groups i.e., MTA, MTA-S, MTA-B (p > 0.05). However, there was higher compressive strength in the MTA-B group when compared to MTA and MTA-S. Also, there was no statistical significant difference between BD, BD-S, BD-B (p>0.05).

Conclusion: This study showed that the compressive strength of MTA and biodentine was not adversely affected by contamination with oral tissue fluids like blood and saliva.


Microleakage, Periradicular tissues, Tricalcium silicate

How to cite this article :

Divya Subramanyam, Madhusudhan Vasantharajan. EFFECT OF ORAL TISSUE FLUIDS ON COMPRESSIVE STRENGTH OF MTA AND BIODENTINE: AN IN VITRO STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 April [cited: 2017 Jun 27 ]; 11:ZC94-ZC96. Available from

DOI and Others

DOI: 10.7860/JCDR/2017/24510.9722


Date of Submission: Apr 10, 2016
Date of Peer Review: Oct 20, 2016
Date of Acceptance: Jan 09, 2017
Date of Publishing: Apr 01, 2017

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