Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 22930

Original article / research
Year : 2017 | Month : April | Volume : 11 | Issue : 4 | Page : ZC16 - ZC19

Antibacterial Effects of Garlic Extracts and Ziziphora Essential Oil on Bacteria Associated with Peri-Implantitis

Farzane Pakdel, Shima Ghasemi, AmirReza Babaloo, Yousef Javadzadeh, Rosa Momeni, Milad Ghanizadeh, Seyyed Reza Moaddab, Farzad Yeganeh fathi

1. Assistant Professor, Department of Oral Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran. 2. Assistant Professor, Department of Prosthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran. 3. Assistant Professor, Department of Periodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran. 4. Professor, Department of Pharmaceutical Technology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. 5. Postgraduate Student, Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran. 6. Postgraduate Student, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran. 7. Associate Professor, Department of Laboratory Science, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, Iran. 8. Private Consultant, Biotecnology and Agricultu

Correspondence Address :
Dr. Shima Ghasemi,
Assistant Professor, Department of Prosthodontics, Faculty of Dentistry,
Tabriz University of Medical Sciences, Azadi Street, Tabriz-2435-7654, Iran.
E-mail: dr_shimaghasemi@yahoo.com

Abstract

Introduction: Peri-implantitis is an inflammatory process, characterized by loss of bone around implants and bleeding on probing. Colonization of bacteria in the pocket created around the implant is one of the most important aetiologic factors involved. Although antibiotics are routinely used and are effective agents against this bacterial infection, due to the side effects and drug resistance of these medications, researchers are more interested in using herbal derivatives. There are relatively limited number of studies in this respect.

Aim: To evaluate and compare the antibacterial effects of the extracts of Allium sativum and Ziziphora clinopodioides essential oil on S. aureus and P. aeruginosa.

Materials and Methods: In this in vitro study conducted at Tabriz University of Medical Sciences between March 2016 and July 2016, aqueous and methanolic extracts of garlic and ziziphora essential oil were prepared and then their effects on one standard strain of P. aeruginosa and two standard strains of S. aureus and 18 clinical strains, (nine strains of P. aeruginosa and nine strains of S. aureus) which had been isolated from wound and blood cultures, were evaluated using the reference broth macro dilution method and disk diffusion technique. Data were evaluated with descriptive statistical techniques and t-test for independent groups, using SPSS 17.

Results: Aqueous and methanolic extracts of garlic did not exhibit inhibitory effects on S. aureus and P. aeruginosa. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for ziziphora essential oil against S. aureus were 0.25 and 0.5 µg/mL, respectively. The mean diameter of growth inhibition zone for S. aureus in the ziziphora essential oil group (0.25µg/mL) and Vancomycin group (10 µg/mL) were 26.09±5.8 and 14.63±1.20 mm respectively (p<0.001). Growth inhibition zone for P. aeruginosa were observed in only one (35 mm) and three cases (12.5±3.4 mm) in the ziziphora essential oil group (0.5 µg/mL) and Nalidixic acid group (10 µg/mL) respectively.

Conclusion: The essential oil of ziziphora exhibited a favourable antibacterial effect on S. aureus; therefore, its extract can be used as an effective antimicrobial agent alone or in association with other antibiotics in the treatment of infections due to S. aureus.

Keywords

Allium sativum, Ziziphora clinopodioides, Minimum inhibitory concentration, Nalidixic acid, Vancomycin

How to cite this article :

Farzane Pakdel, Shima Ghasemi, AmirReza Babaloo, Yousef Javadzadeh, Rosa Momeni, Milad Ghanizadeh, et al.. ANTIBACTERIAL EFFECTS OF GARLIC EXTRACTS AND ZIZIPHORA ESSENTIAL OIL ON BACTERIA ASSOCIATED WITH PERI-IMPLANTITIS. Journal of Clinical and Diagnostic Research [serial online] 2017 April [cited: 2017 May 30 ]; 11:ZC16-ZC19. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=April&volume=11&issue=4&page=ZC16-ZC19&id=9620

DOI and Others

DOI: 10.7860/JCDR/2017/24786.9620


Date of Submission: Oct 13, 2016
Date of Peer Review: Nov 14, 2016
Date of Acceptance: Jan 06, 2017
Date of Publishing: Apr 01, 2017

Financial OR OTHER COMPETING INTERESTS: None.

JCDR is now Monthly and more widely Indexed .
  • PubMed Central® (PMC)New
  • Academic Search Complete Database
  • Chemical Abstracts Service
  • Directory of Open Access Journals (DOAJ)
  • EBSCOhostNew
  • Embase & EMbiology
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Open J-Gate
  • Popline (reproductive health literature)
  • SCOPUS
  • www.omnimedicalsearch.com