Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2016 | Month : May | Volume : 10 | Issue : 5 | Page : ZC52 - ZC57

Comparison of Myofibroblasts Between Solid/Multicystic Ameloblastoma and Unicystic Ameloblastoma: An Immunohistochemical Analysis

Gowdara Prakash Smitha, Sadhana Shenoy, Thondikulam Venakataraman Narayan, Ranjita Jayaram

1. Senior Lecturer, Department of Oral Pathology, Rajarajeswari Dental College and Hospital, Bangalore, India. 2. Reader, Department of Oral Pathology, The Oxford Dental College, Hospital and Research Centre, Bangalore, India. 3. Professor and Ex- HOD, Department of Oral Pathology, The Oxford Dental College,Hospital and Research Centre, Bangalore, India. 4. Senior Lecturer, Department of Oral Pathology, KLE Dental College & Hospital, Bangalore, India.

Correspondence Address :
Dr. Gowdara Prakash Smitha,
Gokulam Apartments, B4-313, Doddakalsandra, Kanakpura Main Road, Bangalore-62, India.
E-mail: smithagp24@gmail.com

Abstract

Introduction: Microenvironment is crucial for the maintenance of cellular functions and tissue integrity suggesting that cancer-induced changes in the stroma may contribute to cancer invasion and its biological behaviour. One of the major constituent of the tumour stroma is myofibroblasts. Myofibroblasts are differentiated host fibroblasts that express a-Sma as cytoplasmic microfilaments. They are considered as one of the modified stromal component which in recent years have been thought to have a role in the invasion and aggressive behaviour of odontogenic tumours too.

Aim: To detect immunohistochemically the presence of myofibroblasts in solid/multicystic ameloblastoma and in unicystic ameloblastoma and to see if a relationship exists between the frequency and pattern of distribution of myofibroblasts and the behaviour of ameloblastomas.

Materials and Methods: Ten cases each of solid/multicystic ameloblastoma and unicystic ameloblastoma were stained immunohistochemically for vimentin, a-sm a and desmin. The frequency and pattern of distribution of myofibroblasts in the two study groups were analysed and then compared with clinical and radiographic features of pain and cortical perforation respectively.

Results: Immunohistochemical reaction for a-SMA (alpha Smooth Muscle Actin) showed positive cells in the stroma of both solid/multicystic and unicystic ameloblastomas. The mean number of myofibroblasts was more in unicystic ameloblastoma (UA) compared to Solid/Multicystic Ameloblastoma (SMA). Myofibroblasts expression was dense and arranged in the form of fascicles with indistinct cell borders in one case of follicular ameloblastoma, two cases of plexiform ameloblastoma and in a focal area of one case of type 1UA. In all other cases where the expression was noted, the myofibroblasts were spindle in shape with distinct cell boundaries.

Conclusion: The results of the study indicate that myofibroblasts alone may not play a role in the behaviour of ameloblastomas. This calls for determining the role of various other stromal components in the biological behaviour of ameloblastomas. Our study could not establish a correlation between pattern of distribution of myofibroblasts and the behaviour of ameloblastomas.

Keywords

Biological behaviour, Cortical perforation, Pain, Stromal patterns

How to cite this article :

Gowdara Prakash Smitha, Sadhana Shenoy, Thondikulam Venakataraman Narayan, Ranjita Jayaram. COMPARISON OF MYOFIBROBLASTS BETWEEN SOLID/MULTICYSTIC AMELOBLASTOMA AND UNICYSTIC AMELOBLASTOMA: AN IMMUNOHISTOCHEMICAL ANALYSIS. Journal of Clinical and Diagnostic Research [serial online] 2016 May [cited: 2018 Jun 23 ]; 10:ZC52-ZC57. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2016&month=May&volume=10&issue=5&page=ZC52-ZC57&id=7778

DOI and Others

DOI: 10.7860/JCDR/2016/14380.7778


Date of Submission: Apr 09, 2015
Date of Peer Review: Jun 14, 2015
Date of Acceptance: Dec 31, 2015
Date of Publishing: May 01, 2016


Financial OR OTHER COMPETING INTERESTS: None.

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