Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2016 | Month : May | Volume : 10 | Issue : 5 | Page : ZC106 - ZC111

Histopathological and Radiographic Analysis of Dental Follicle of Impacted Teeth Using Modified Gallego’s Stain

Evie satheesan, Sandhya Tamgadge, Avinash Tamgadge, Sudhir Bhalerao, Treville Periera

1. Post Graduate Student, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital, Navi Mumbai, Maharashtra, India. 2. Professor and PG Guide, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital, Navi Mumbai, Maharashtra, India. 3. Professor and HOD, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital, Navi Mumbai, Maharashtra, India. 4. Professor and PG Guide, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital, Navi Mumbai, Maharashtra, India. 5. Professor and PG Guide, Department of Oral and Maxillofacial Pathology and Microbiology, Padmashree Dr D Y Patil Dental College and Hospital, Navi Mumbai, Maharashtra, India.

Correspondence Address :
Dr. Sandhya Tamgadge,
Professor and PG Guide, Department of Oral and Maxillofacial Pathology and Microbiology,
Padmashree Dr D Y Patil Dental College and Hospital, Sector 7, Nerul, Navi Mumbai, Maharashtra, India.
E-mail: sandhya.tamgadge@gmail.com

Abstract

Introduction: In the WHO classification of odontogenic tumours, hard tissue formation has been considered as a sub-classification however, this parameter has not been much explored in dental follicle in literature. Epithelial-mesenchymal interactions play an important role in odontogenesis and its associated pathologies; therefore research on dental follicle should also include mesenchymal components along with epithelial components. Additionally, special stains to identify the nature of such depositions in dental follicle have been less explored. Modified Gallego’s stain is such an example which has not been tried in odontogenic lesions which makes this study unique.

Aim: Aim of this study was to study histopathological variations in dental follicle, the nature of calcification and depositions using Modified Gallego’s stain and to correlate histological features of dental follicle with pericoronal width radiographically.

Materials and Methods: A prospective histological study of the dental follicles of 50 impacted teeth was carried out to microscopically evaluate the dental follicular tissues for pathological changes, and to correlate it with pericoronal radiolucency. Impacted teeth with pericoronal radiographic width less than 3mm were included in the study and symptomatic teeth were excluded. Further Modified Gallego stain was used to differentiate the nature of hard tissue formation in dental follicle tissues.

Results: Dental follicle histologically showed pathological changes resembling dentigerous cyst, ameloblastoma, odontogenic fibroma (Simple and WHO Type), clear cell odontogenic tumour, neurofibroma, neurilemmoma and mucoepidermoid carcinoma.

Conclusion: The dental follicle surrounding an impacted tooth has the potential to differentiate into a wide variety of tissue types, and thus shows the potential for cyst and tumour development which was observed in this study in most of the specimens with normal follicular width radiographically.

Keywords

Benign neoplasms, Dental follicle, Odontogenic tumours, Odontogenic cysts, Oral pathology, Pathologies, Radiographic width

How to cite this article :

Evie satheesan, Sandhya Tamgadge, Avinash Tamgadge, Sudhir Bhalerao, Treville Periera. HISTOPATHOLOGICAL AND RADIOGRAPHIC ANALYSIS OF DENTAL FOLLICLE OF IMPACTED TEETH USING MODIFIED GALLEGO’S STAIN. Journal of Clinical and Diagnostic Research [serial online] 2016 May [cited: 2018 Jul 21 ]; 10:ZC106-ZC111. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2016&month=May&volume=10&issue=5&page=ZC106-ZC111&id=7838

DOI and Others

DOI: 10.7860/JCDR/2016/16707.7838


Date of Submission: Sep 09, 2015
Date of Peer Review: Oct 30, 2015
Date of Acceptance: Feb 28, 2016
Date of Publishing: May 01, 2016


Financial OR OTHER COMPETING INTERESTS: None.

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