Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 3821

Original article / research
Year : 2016 | Month : May | Volume : 10 | Issue : 5 | Page : DC01 - DC05

Prevalence of Class D Carbapenemases among Extended-Spectrum -Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord

Mohammad-Sadegh Damavandi, Abolfazl Gholipour, Mohammad Latif pour

1. Student, Department of Microbiology and Immunology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. 2. Assistant Professor, Department of Microbiology and Immunology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. 3. Student, Department of Microbiology and Immunology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Correspondence Address :
Dr. Abolfazl Gholipour,
Assistant Professor, Department of Microbiology and Immunology, Cellular and Molecular Research Center,
Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
Email: gholipour_abolfazl@yahoo.com

Abstract

Introduction: Extended-spectrum -lactamases (ESBLs) are a set of plasmid-borne, various and quickly evolving enzymes that are a main therapeutic issue now-a-days for inpatient and outpatient treatment.

Aim: The aim of this study was to determine multi-drug resistance (MDR) and ESBLs producing E. coli strains, prevalence of class D Carbapenemases among ESBLs producing Escherichia coli isolates from educational hospitals in Shahrekord, India.

Materials and Methods: Uropathogenic Escherichia coli strains were isolated from patients with Urinary Tract Infections (UTIs). The agar disc diffusion test was used to characterize the antimicrobial sensitivity of the E. coli isolates. The ESBL positive strains were identified by phenotypic double-disk synergy test, by third-generation cephalosporin in combination with or without clavulanic acid. Multiplex PCR was carried out for detection of the three families of OXA-type carbapenamases including OXA-23, OXA-24, and OXA-48 in E. coli strains.

Results: All bacterial isolates were susceptible to meropenem. Ninety isolates produced ESBL, 55 E. coli isolates from inpatients, and 35 isolates from outpatients, with a significant association (p< 0.05). The prevalence of OXA-23, OXA-24, and OXA-48 in the ESBLs producing isolates was respectively 21%, 18%, and 11% for inpatients, and 10%, 8%, and 6% for outpatients.

Conclusion: ESBL-producing E. coli isolates are also a major threat in the clinical setting. The findings of this study indicated the high occurrence of ESBLs and multiple antibiotic resistance in E. coli isolates.

Keywords

Bacterial resistance, Multiplex polymerase chain reaction, Urinary tract infections

How to cite this article :

Mohammad-Sadegh Damavandi, Abolfazl Gholipour, Mohammad Latif pour. PREVALENCE OF CLASS D CARBAPENEMASES AMONG EXTENDED-SPECTRUM -LACTAMASES PRODUCING ESCHERICHIA COLI ISOLATES FROM EDUCATIONAL HOSPITALS IN SHAHREKORD. Journal of Clinical and Diagnostic Research [serial online] 2016 May [cited: 2018 Apr 25 ]; 10:DC01-DC05. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2016&month=May&volume=10&issue=5&page=DC01-DC05&id=7739

DOI and Others

DOI: 10.7860/JCDR/2016/17722.7739

Date of Submission: Nov 06, 2015
Date of Peer Review: Dec 13, 2015
Date of Acceptance: Jan 06, 2016
Date of Publishing: May 01, 2016


Financial OR OTHER COMPETING INTERESTS: None.

JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2016: 132.37
  • SCOPUS
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • EBSCOhost
  • Embase & EMbiology
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)
  • www.omnimedicalsearch.com